Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/15/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Arthropod was found in the school garden in clusters on the stems of milkweed in front of classroom. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Aphididae |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | Both gels ran for 35 minutes at 135 volts and 70 amps. Results were viewed w/ a white light box. *Wells starting new ruler (Gel 1: Arthropod) Lane 1: DNA Ladder Lane 2: Aphids DNA Lane 3: Ants DINA Lane 4: (+) Arthropod Control Lane 5: (-) Arthropod Control Lane 6: DNA Control Lane 7: Water
*Wells starting new ruler (Gel 2: Wolbachia) Lane 1: DNA Ladder Lane 2: Aphids DNA Lane 3: Ants DINA Lane 4: (+) Wolbachia Control Lane 5: (-) Wolbachia Control Lane 6: DNA Control Lane 7: Water |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Both gels did not detect visual evidence of bands when running gel electrophoresis. After consideration of the protocol, the error must have occurred in the PCR process for the DNA controls and ladder were not apparent in their designated lanes. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman