Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations |
|
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Although we had sterile technique, multiple people in the lab were using the same materials like ladders, buffer, dyes, etc. which could’ve eventually led to some type of mishap in the lab. The controls did work so we know we can trust the results for the most part. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coccinellidae was found to be postive for Wolbachia. |
Water Boatman