Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Arthropod was captured in the school garden infront of the classroom on one of the plant leaves. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | The gel ran for about 35 minutes at 135 volts and 70 amps. Results were viewed using a white light box. Lane 1: DNA Ladder Lane 2: Spotted ladybug DNA Lane 3: No spots ladybug DINA Lane 4: (+) Arthropod Control Lane 5: (-) Arthropod Control Lane 6: DNA Control Lane 7: Water
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Was unable to successfully run gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis