GRP11 – Coccinellidae

May 25, 2024

Sample information
Picture
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Arthropod was captured in the school garden infront of the classroom on one of the plant leaves.
Putative identification Arthropoda

Methods
Extraction kit tweezers
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

The gel ran for about 35 minutes at 135 volts and 70 amps. Results were viewed using a white light box.

Lane 1: DNA Ladder

Lane 2: Spotted ladybug DNA

Lane 3: No spots ladybug DINA

Lane 4: (+) Arthropod Control

Lane 5: (-) Arthropod Control

Lane 6: DNA Control

Lane 7: Water

 

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Was unable to successfully run gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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