Chaetocnema hortensis

May 27, 2024

Sample information
Picture
Photos by: Ilayda and Marisa
Location
Collection date 08/25/2023
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

non gel image available

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCTATCAGGTAATGCTGAGTA CTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTAC ACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGA TTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGG GTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGTGGGAACTTCAATAAGAATTTTAATTCGAGCAGAATTAGGAAGCCCAGGATCCTTAATTGGAAATGATCAAATTTATA ATGTAATTGTTACTGCTCATGCATTTGTAATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAATTGA TTAGTTCCATTAATAATTGGAGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTCTGGCTTTTACCCCCCTC TTTATTTTTATTACTAATAAGAAGATTAGTAGAAAGAGGAGCTGGAACAGGATGAACAGTTTACCCCCCACTTTCATCAA ATATTGCTCATGGTGGATCTTCAGTTGATCTCGCAATTTTTAGACTCCATTTAGCAGGAGTTTCTTCTATTTTAGGAGCA ATTAATTTTATTACAACAATTATTAATATACGACCTCAAGGAATATCTTTTGATCAAATACCCTTATTTGTATGGGCAGT ACTAATTACTGCTATTCTTTTACTTTTATCACTACCAGTTTTAGCTGGAGCTATTACAATACTTTTAACTGACCGAAATT TAAATACATCATTTTTTGACCCAATTGGTGGGGGAGATCCTATCCTATACCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be postive for Wolbachia.
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