Tetragnatha montana

May 27, 2024

Sample information
Picture
Photos by: Ilayda and Marisa
Location
Collection date 08/25/2023
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae Agelenidae

Methods
Extraction kit Instagene Matrix
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

no gel image available

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

amplifiation was done twice
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TATAGTAGGGACTGCTATAAGAGTTTTAATTCGAATTGAATTAGGTCAATCTGGAAGATTTTTAGGAGATGATCAGTTAT ACAATGTTATTGTTACTGCTCATGCATTTGTTATAATTTTTTTTATAGTGATACCAATTTTAATTGGTGGATTCGGTAAT TGGTTGGTTCCTTTAATGTTAGGAGCTCCAGATATGGCTTTTCCTCGGATAAATAATTTAAGTTTTTGACTTCTTCCCCC CTCTCTTTTTATATTATTTATTTCTTCTATAGTAGATATAGGGGTTGGGGCAGGATGAACTGTTTATCCTCCTTTAGCTT CTTTAGAAGGACACCCAGGTAGATCTGTTGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCTTCTATTATAGGG GCTATTAATTTTATTTCCACTATTATTAATATACGAATAAGAGGAATTTCTATAGAAAAGGTCCCACTTTTTGTGTGATC TGTCCTAATTACTGCTGTTTTATTACTATTATCTTTACCTGTTTTAGCAGGTGCTATCACAATATTGTTAACGGATCGAA ATTTTAATACTTCTTTTTTTGATCCTTCTGGAGGAGGGGATCCAGTGTTGTTTCAACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Agelenidae was found to be negative for Wolbachia.
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