Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Kohler High School |
| Observations | Curled up and smaller in stature |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Qiagen Blood and Tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Two ladders used. One is a benched up 100 BP ladder from ProMega. The other one is HindIII Lambda digest DNA standard. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No glow appeared matching the Wolbachia negative control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis