Mosquito

June 3, 2024

Sample information
Picture
Photos by: Aiden C.
Location
Collection date 12/13/2023
Captive / Cultivated? Wild-caught
Group Kohler High School
Observations

Long proboscis
Putative identification Arthropoda

Methods
Extraction kit Qiagen Blood and Tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Two ladders used. One is a benched up 100 BP ladder from ProMega. The other one is HindIII Lambda digest DNA standard.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Mosquito band matches the Wolbachia positive control well in brightness.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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