Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/01/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Talladega College |
| Observations | Female |
| Putative identification | Arthropoda Chelicerata Arachnida Ixodida Ixodidae Dermacentor Dermacentor variabilis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | PCR run with Wolbachia positive DNA and DNA from Wolbachia positive fruit flies |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
NNNAANCTTATTTTCGGCAGTTCGAGCAGNGAGTCACNAGGAATAAGAATAAGAATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTAATTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCATTCATATAAGGAGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTATCACCTCCATCATTATTTTTACTTATAANTTCTTCATTAATTGAATCAGGAGCAGGAACAGGTAGAACTGTTTATCCCCCTTTATCAACAAATTTGTCTCACTATGGACCTGCAGTTGATTCAGCTATTTTTAGCCTTCATCTAGCAGGTGCTTCCACTTTTTAAGGAGCGATTAATTTTATCACCACATTAATAAATAAACGATCAATTGGTTTAACTCTGGAACGAATACTTTTTTTTGTTTGAGCAGTTTTAATAACAGCTATTTTACTTCTTCTTTCTCTCCTTTTTTAAGCAGGAGCCATAACAATTTTATAAACAGACCGTAACTTTAATACTTCCTTTTTCNACCCTGCAGGGGGGGAAGACCCCTTTTTGTATCACCTTTTTTTTTTGNTTTTTTGGCACCAAAAAAAAATAAAAAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Dermacentor variabilis was found to be negative for Wolbachia. |
Water Boatman