Hypnorna sp.

July 21, 2024

Sample information
Picture
Photos by: Andy C.
Location
Collection date 09/10/2024
Captive / Cultivated? Wild-caught
Group Talladega College
Observations
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

PCR ran with Wolbachia DNA control and DNA from Wolbachia positive fruit flies.
Wolbachia 16S sequence Download AB1
GGGGGGTCCGGAATTCCAACNAGTGTTGCATGGCTGTCGTCAGCTCGTGTTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGTAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACCTCGAAGCTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
GTAAACCNAANTTGGAACTGATCTGGATAGTGGGACTTCATTAAGAATATTAATTCGAGCCGAATTAAATCAACCAGGATCACTAATTGGAGATGACCAAATTTATAATGTTATTGTTACAGCTCACGCTTTTATTATAATTTTCTTTATAGTTATACCTATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGAGCACCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGATTATTACCACCATCACTTTCATTATTATTAACAAGTAGTATGGTTGAAATAGGAGCTGGCACTGGATGAACCGTTTACCCCCCTCTAGCTAGAGGCATTGCCCATGAAGGGGCATCCGTAGACCTAGCAATCTTTTCATTACATTTAGCAGGAATTTCATCAATTTTAGGTGCAGTAAATTTTATTTCTACTACAATTAAAATAAAACCAAACTATATAAAACCTGATCAAATTCCTTTATTTGTATAAGCAGTNTCAATTACTGCCTTATTATNATAATAATCATTGCCTGTTTTAGCAGGTGCTATTACAATTTTTTTGACAGATCGAAACTTATATACTTCTTTTTTTGATCCTGCGGGTGGGGGTGATCCATTTTTATATCAACATNNTTCTGATTTTTGACAACACCAAAAAAAAAAAAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be postive for Wolbachia.
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