Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 07/01/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Talladega College |
| Observations | Female |
| Putative identification | Arthropoda Chelicerata Arachnida Ixodida Ixodidae Dermacentor Dermacentor variabilis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | PCR ran with Wolbachia DNA control and DNA from Wolbachia positive fruit flies. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
ACAACCACCCTCGGAGTTGAGCAGGANCACAGGATAAGAATAAGATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTTAGTTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCAATTATATTAAGGAGGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCATTATTTTTACTTATAAATTCTTCATTAATTGAATCAGGAGCAGGAACAGGTTGAACTGTTTATCCCCCTTTATCATCAAATTTGTCTCATTATGGACCTTCAGTTGATTTAGCTATTTTTAGCCTTCATCTAGCAGGTGCTTCTACTATTTAAGGAGCGATTAATTTTATTACCACAATTTTTAATACACGNCCAATTGGTTAAACTTTTGAACGAATACTTTTATTTGTTTGATCAGTTTTAATTACAGCAATTTTACTTCTCCTTCTCTCCTGTTTTAGCAGGAGCAATAACAAATTATTAAACAGACCGAAATTTANNACCTCCTTTTTGACCNTCCAGGTGGTGGAGACCCCTTTTGTTGCACCTCCACNTTAGATTTTTGTCACCNNNNNTNAAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Dermacentor variabilis was found to be negative for Wolbachia. |
Spotted cucumber beetle (Diabrotica undecimpunctata) C-5 SSP
DKH10
DKH 9
DKH8
DKH 7