Sample information |
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Picture |
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Location | |
Collection date | 07/01/2022 |
Captive / Cultivated? | Wild-caught |
Group | Talladega College |
Observations | Female |
Putative identification | Arthropoda Chelicerata Arachnida Ixodida Ixodidae Dermacentor Dermacentor variabilis |
Methods |
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Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | GelRed |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | PCR ran with Wolbachia DNA control and DNA from Wolbachia positive fruit flies. |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download AB1
ACAACCACCCTCGGAGTTGAGCAGGANCACAGGATAAGAATAAGATTTTAATTCGAATAGAATTAAGCCAACCAGGAACTTTTAGTTGGAAATGATCAAATTTATAACGTAATTGTAACTGCTCATGCATTTATTATAATTTTTTTCATAGTTATGCCTATCATAATTGGTGGGTTTGGAAACTGACTTGTACCAATTATATTAAGGAGGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCATTATTTTTACTTATAAATTCTTCATTAATTGAATCAGGAGCAGGAACAGGTTGAACTGTTTATCCCCCTTTATCATCAAATTTGTCTCATTATGGACCTTCAGTTGATTTAGCTATTTTTAGCCTTCATCTAGCAGGTGCTTCTACTATTTAAGGAGCGATTAATTTTATTACCACAATTTTTAATACACGNCCAATTGGTTAAACTTTTGAACGAATACTTTTATTTGTTTGATCAGTTTTAATTACAGCAATTTTACTTCTCCTTCTCTCCTGTTTTAGCAGGAGCAATAACAAATTATTAAACAGACCGAAATTTANNACCTCCTTTTTGACCNTCCAGGTGGTGGAGACCCCTTTTGTTGCACCTCCACNTTAGATTTTTGTCACCNNNNNTNAAA
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Dermacentor variabilis was found to be negative for Wolbachia. |