Trachelipus rathkii TS2

September 10, 2024

Sample information
Picture
Photos by: Tabita S.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda Trachelipodidae Trachelipus

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level
Explanation of confidence level

CO – 708 bp was visible

16SrRNA – 438 bp wasn’t visible
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TACAGCAATTCTCTTATTACTTTCACTACCGGTTTAGCAGGGGCCATTACTATRTTATTAACAGACCGTAATTTGAACACCTCTTTTTTCGACCCTAGGGAG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Trachelipus was found to be negative for Wolbachia.
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