Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Myrmica Myrmica rubra |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | |
| Explanation of confidence level | CO – 708 bp was visible 16SrRNA – 438 bp wasn’t visible |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TTAGAATTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATTCCTTTAATATTAGGATCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGTGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGGACCTTCAATTG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Myrmica rubra was found to be negative for Wolbachia. |
Joro Spider
Imported Red Fire Ant
Black carpenter ant
Flocculent tailed millipede
Tawny crazy ant