Myrmica rubra – AFM1

September 11, 2024

Sample information
Picture
Photos by: Luisa H., Anna M.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

At the edge of a damp forest path of a mixed forest, that was partially covered by moss.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Myrmica Myrmica rubra

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

CO – 708 bp –> a visible line

16S rRNA – 438 bp –> no visible line
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Myrmica rubra was found to be negative for Wolbachia.
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