Musca domestica AB2

September 11, 2024

Sample information
Picture
Photos by: Laurin E.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Wald, warm.
Putative identification Arthropoda Hexapoda Insecta Diptera Muscidae Musca Musca domestica

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Confident
Wolbachia 16S sequence Download FASTA    Download AB1
CAATTCTATATTCCGTTCTATCTGGCACATTCGTTGCAAAACTAGGATCGAAAACGGGCATGGCCRCTATTCTAATTATATGTTCGAAGYTGTTTTGGTGATTTTCCGWTTTCACTGCCAACCACCATACAGCAGCTCTGTAGAATAGAATTGGTTTCACTCGAAGCTTATTATTAGTAAGTAGTATAGKAGAAAACGGAGCTGGRACAGGATGAACTGTTTACCCCCCTCTATCTTCAAATATTGCCCATGGTGGAGCTTCTGTTGATTTAGCAATTTTCTCTCTYCACTTAGCAGGAATTTCTTCKAKKCTAGGGGSKGTWAATTTTATTACAACTGTGATTAATATACGYTCMACTGGAATTACATTTGATCGAATACCTTTATTTGTTTGATCAGGTGKKATKACAGCTTTATTACTTCTTTTATCTTTACCAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
WATAGTAGGAACTTCTTTAAGAATTCTTATTCGGGCTGAATTAGGACACCCAGGAGCATTAATTGGAGATGACCAAATTTATAATGTAATTGTTACAGCCCATGCTTTCATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTCCCCCTAATATTAGGAGCCCCTGATATAGCCTTTCCCCGAATAAATAATATAAGTTTTTGACTTTTACCTCCTGCATTAACTTTATTATTAGTAAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACTGTTTACCCCCCTCTATCTTCAAATATTGCCCATGGTGGAGCTTCTGTTGATTTAGCAATTTTCTCTCTTCACTTAGCAGGAATTTCTTCTATTCTAGGGGCTGTAAATTTTATTACAACTGTGATTAATATACGTTCAACTGGAATTACATTTGATCGAATACCTTTATTTGTTTGATCAGTTGTTATTACAGCTTTATTACTTCTTTTATCTTTACCAGTTTTAGCAGGAGCAATTACTATATTATTAACAGATCGAAATTTAAACAYTTCATTTTTTGATCCAGCAGGAGGTGGAGATCCTATTTTATATCAACACTTATTTTGATTTTTTGGTCACT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Musca domestica was found to be postive for Wolbachia.
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