Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/29/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Auburn University at Montgomery |
| Observations | A black carpenter ant found roaming in leaf litter. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | Ethidium Bromide |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Wolbachia was positively identified by both electrophoresis and sequencing. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GGTGTTGnnTGGCTGTCGTCnGCTCGTGTCGTGAGnnGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTT ACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCAT GGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCT TAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACG CCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TTGnNnnTTnnnCGGGnnnAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACnATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATnnTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCnnnTATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAnGnGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTnnTTnnTnnnnCnCTCnGGnnGnnGnnnnnnnAnTTnnTnCnnAnnTTTnnTTnnnnTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Formicidae was found to be postive for Wolbachia. |
Water Boatman