Sample information
Picture	Photos by: Olivia A
Location
Collection date	09/25/2024
Captive / Cultivated?	Captive / Cultivated
Group	Palm Beach Atlantic University
Observations	This cricket was kept in captivity, primarily used to feel reptile pets.
Putative identification	Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus
Methods
Extraction kit	tweezers
DNA extraction location	Gonads dissected
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Acheta domesticus was found to be postive for Wolbachia.