House cricket

October 22, 2024

Sample information
Picture
Location
Collection date 09/26/2024
Captive / Cultivated? Captive / Cultivated
Group Palm Beach Atlantic University
Observations

Purchased from Petco via brand Timberline Fresh.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

The PCR products were run along a ThermoFisher Scientific GeneRuler 100 bp Ladder on a 2% agarose gel, as shown in table 3 (ThermoFisher Scientific 2024). 1X TAE was used in the gel and for running buffer, with 20,000X SmartGlow, a BioRad electrophoresis chamber and FisherScientific power source. The gel was run at 100 volts for 35 minutes and visualized using a Spectroline Ultraviolet Transilluminator.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The first arthropod used for DNA extraction in lane 2 did not produce bands from the PCR products, presumably due to lack of grinding the abdominal tissues thoroughly. The second arthropod in lane 3 produced one band, the CO1 gene fragment. It did not produce the 16s rRNA gene fragment. However, these crickets were bred by the company Timberline Fresh and had not been previously been exposed to the natural environment. The parents presumably did not either.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Acheta domesticus was found to be negative for Wolbachia.
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