Pill Bug – Armadillidium nasatum

November 3, 2024

Sample information
Picture
Photos by: Alex A.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

I found this pill bug in a rotting log by a trail path in the Olmstead Woods in the morning. There were other pill bugs in the same area. This pill bug is a little bit smaller than most pill bugs I’ve seen.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium nasatum

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All our controls worked as expected and the results matched the gel electrophoresis.
Wolbachia 16S sequence Download FASTA    Download AB1
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGGCTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CGCTTTAAGAATAATTATTCGGACTGAATTAGGGCAGCCTGGGAGTCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGTAATGATTTTCTTCATAGTAATACCTATTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTAGGGGCTCCTGATATAGCTTTCCCTCGTATAAACAATATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAGCAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAGCTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACTATAATTAATATACGGGCGTCCGGAATTAGTATGGATCGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTATTACTATACTATTGACTGACCGTAATTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium nasatum was found to be postive for Wolbachia.
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