Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | This arthropod was found outside near my front porch on a cool night. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae Dichaetophora |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked as expected, and other members in our group were able to identify the presence of Wolbachia, whereas I could not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
CNTTTTGGGGCNTGAGCCGGAATGGTTGGAACTTCTTTAAGAATTTTAATCCGAGCAGAATTNGGGNN NCCTGGGGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCTCACGCTTTTGTAATAATTTTTTTTATAG TTATACCAATTATAATTGGAGGATTTGGAAATTGACTACTTCCTTTAATATTAGGAGCTCCCGATATCGCTNTTCCNCGA ATAAATAATATAAGTTTCTGACCGCTTCCCCCTTCTNTTACTTTACTTTTAGTANGTNNAATANTTGAAAACGGAGCGGG GACACGGTGAACTGCCTATCCCCCCCCCTCNCCTCCCCTNGCNCATGGAGGGCCTTCCATAAATTTAGCTCTTTTTANAC TTCNTTTNNCNCGANNCTATTCCGNTTNGGAGCTGAAAATNNCCCTACTCCACTTACTAATAGACGATCAACNGGAATCA CNTTTGATCTGATACCNTTATTTGTATGATCNCTTGCTATTACTGCTTTATTACTCCTTCCCTCTNNACCTGATCTANAN GGANCTATTACCATGTTATTAACAGANANNAATTTANTTACTTCATTTTTTGANCCTGCNNGAGGANGACATCCTTNTTT TTATTNANNTNNNTTTNGACTNTTTTGGTCACCCNNAAANTTTAAN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Dichaetophora was found to be negative for Wolbachia. |
Water Boatman