Dichaetophora sp. WTS5

November 8, 2024

Sample information
Picture
Photos by: Andrés C.-V., Dr. Kerry O.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

This arthropod was found outside near my front porch on a cool night.
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Dichaetophora

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked as expected, and other members in our group were able to identify the presence of Wolbachia, whereas I could not.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CNTTTTGGGGCNTGAGCCGGAATGGTTGGAACTTCTTTAAGAATTTTAATCCGAGCAGAATTNGGGNN NCCTGGGGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCTCACGCTTTTGTAATAATTTTTTTTATAG TTATACCAATTATAATTGGAGGATTTGGAAATTGACTACTTCCTTTAATATTAGGAGCTCCCGATATCGCTNTTCCNCGA ATAAATAATATAAGTTTCTGACCGCTTCCCCCTTCTNTTACTTTACTTTTAGTANGTNNAATANTTGAAAACGGAGCGGG GACACGGTGAACTGCCTATCCCCCCCCCTCNCCTCCCCTNGCNCATGGAGGGCCTTCCATAAATTTAGCTCTTTTTANAC TTCNTTTNNCNCGANNCTATTCCGNTTNGGAGCTGAAAATNNCCCTACTCCACTTACTAATAGACGATCAACNGGAATCA CNTTTGATCTGATACCNTTATTTGTATGATCNCTTGCTATTACTGCTTTATTACTCCTTCCCTCTNNACCTGATCTANAN GGANCTATTACCATGTTATTAACAGANANNAATTTANTTACTTCATTTTTTGANCCTGCNNGAGGANGACATCCTTNTTT TTATTNANNTNNNTTTNGACTNTTTTGGTCACCCNNAAANTTTAAN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Dichaetophora was found to be negative for Wolbachia.
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