DERMAHF24-AN Dermaptera

November 8, 2024

Sample information
Picture
Photos by: Ashlea N.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Kinsey (past lab partner) and I collected insects on August 27, 2024 
  • Insects were collected from 1-1:30pm, my specific insect sample DERMAHF24-AN, was collected around 1:23
  • Most insects were collected from the ‘International Gardens’ across from The Learning Commons on campus 
  • Most insects collected were inhabiting shrubs or grass, however some insects were collected airborne 
  • Insects were collected using a net and jar. Kinsey operated the net and forceps, while I enclosed the insects in the jar when captured 
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I predicted that Wolbachia would not be present, and I extracted good quality DNA that was used during PCR and later during gel electrophoresis it was proven no Wolbachia was found.
Wolbachia 16S sequence
Arthropod COI sequence
TGGATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATC CCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTA GTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGA GGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGC ATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTAT TTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGA ACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGT AGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAG GATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATA AATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAGCAAT TTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTAT TATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGA GATCCAATTCTTTATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Dermaptera was found to be negative for Wolbachia.
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