Sample information |
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Picture |
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Location | |
Collection date | 08/27/2024 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations |
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Putative identification | Arthropoda Hexapoda Insecta Dermaptera |
Methods |
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Extraction kit | |
DNA extraction location | Rear half |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
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Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | I predicted that Wolbachia would not be present, and I extracted good quality DNA that was used during PCR and later during gel electrophoresis it was proven no Wolbachia was found. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
TGGATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATC CCCAGGATCATGAATTAGCAATGATCAAATTTATAATACAATTGTTACTA GTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGA GGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGC ATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTAT TTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGA ACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGT AGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAG GATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATA AATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAGCAAT TTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTAT TATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGA GATCCAATTCTTTATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Dermaptera was found to be negative for Wolbachia. |