Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Captured in the corner of a room in a building during the summer. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Pholcidae Pholcus Pholcus crypticolens |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | All our controls worked as expected and the results matched the gel electrophoresis. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATTAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence | Download FASTA
Download AB1
TTTAATATTAGGAGCTCCAGATATAGCGTTTCCTCGGATAAATAACTTGAGATTTTGGTTATTACCTCCTGCTGTTTTATTATTANTGTTGTCTGGTTTGGTGGAAACTGGAGTANGGGCGGGGTGGACTATTTATCCCCCGTTATCTTCTANAGTANGACATANCGGAGTGTCAATAGATTTTGCTATTTTTTCTCTTCATTTAGCAGGGGCTTCTTCTATTATAGGAGCTATTAACTTTATTTCTACTGTTATAAATATACGTTTAATAGGGGTGGGTATAGATAAAATTAGCTTATTTGTATGATCAGTGGTGATTACGGCGGTTTTANTANTACTTTCTTTACCCGTATTAGCTGGAGCAATNACTATACTTTTGACTGA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Pholcus crypticolens was found to be postive for Wolbachia. |
Water Boatman