Coreus marginatus_ZuercherUnterland_smiley

October 25, 2024

Sample information
Picture
Photos by: Julia C. Svenia H.
Location
Collection date 08/19/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Caught on the leaf of a raspberry bush, frozen to death and stored in alcohol until DNA-extraction.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Coreidae Coreus Coreus marginatus

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

ArthropodPCR-Gel: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 8.

As seen from the lack of band, something clearly went wrong. It is most likely that this happened in the sample preparation phase since even after repeating the PCR-process nothing showed up. This sample is, therefore, unusable.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

We are confident that we have no clue if the sample was infected since the PCR failed to produce results even for the arthropod gene.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary
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