Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | Caught on the leaf of a raspberry bush, frozen to death and stored in alcohol until DNA-extraction. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Coreidae Coreus Coreus marginatus |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | ArthropodPCR-Gel: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 8. As seen from the lack of band, something clearly went wrong. It is most likely that this happened in the sample preparation phase since even after repeating the PCR-process nothing showed up. This sample is, therefore, unusable. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We are confident that we have no clue if the sample was infected since the PCR failed to produce results even for the arthropod gene. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | |
Water Boatman