Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | The mosquito was found in a leafy area near an oak tree in Rock Creek Park. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | DNA extraction: I made sure to crush the arthropod very well. Then, I dissected the abdomen and did not include the exoskeleton. All of the lanes are labeled on the gel image Lanes 9 and 10 are reruns of an originally unsuccessful attempt for a classmate’s Pill Bug The Wolbachia bands were 438bp each The CO1 bands were 708bp each On the CO1 gel, my arthropod is labeled, along with the positive CO1 control and the 100bp ladder. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | It is clear that the arthropod this sequence corresponds to has Wolbachia. However, we did not get a match for the same arthropod that I extracted DNA from (mosquito). The arthropod that we got from the sequence of nucleotides was a Roly Poly bug. This could have been a contamination error during the extraction or transportation process. |
| Wolbachia 16S sequence | Download FASTA
GCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTnGCnATCAGGTAATGCTGAnTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGTTGCAAnGTGCGCAAGCCTAnnCTAnTCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACnTGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
CTGGGAGTCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGnAATGATTTTCTTCATAGTAATACCTATTATAATTGGANGTTTTGGAAATTGATTAATTCCNTTGATATTAGGGGCTCCTGATATAGCTTTCNCTCGTATAAACAATATAAGATTTTGGNTGNTTCCCCCNTCTTTAACNTTACTCCTTANCAGANGATTANTTGAAANAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCNTCNNNAATTGCCCATNNTGGAGCTTCTGTAGATTTAGGTATCTTTTNTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGNAGTAAACTTTATTACNACTNTNATTAATATACGNGCNTCCGGAATTACTATGGATCGTGTGCCTTTATTTGTTTGNTCTGTTTTANTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGNT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Culicidae was found to be postive for Wolbachia. |
Water Boatman