Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | In gras next to stream. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Syrphidae Episyrphus Episyrphus balteatus |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | Reasons why it didn‘t work during the first try (Picture 1): – Foam formation (broken machine) – to much/few sample pipetted in the gel – Centrifuged for too little time |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Episyrphus balteatus (Hainschwebfliege/ Grove Hoverfly)
https://de.wikipedia.org/wiki/Hainschwebfliege DNA tested.
|
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Episyrphus balteatus was found to be postive for Wolbachia. |
Water Boatman