Suillia_affinis_kreis_5

November 14, 2024

Sample information
Picture
Photos by: Leandro P
Location
Collection date 08/18/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

I found it on a grass field next to my house.
Putative identification Arthropoda Hexapoda Insecta Diptera Heleomyzidae Suillia Suillia affinis

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

Reasons why it did not work the first time(Picture 1):

– Foam reaction

– to long in centrifugator

– to much of the sample

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Suillia affinis (Heleomyzidae/Scheufliege)

https://en.wikipedia.org/wiki/Suillia_affinis

DNA tested.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Suillia affinis was found to be negative for Wolbachia.
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