Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/18/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | I found it on a grass field next to my house. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Heleomyzidae Suillia Suillia affinis |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | Reasons why it did not work the first time(Picture 1): – Foam reaction – to long in centrifugator – to much of the sample |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Suillia affinis (Heleomyzidae/Scheufliege)
https://en.wikipedia.org/wiki/Suillia_affinis DNA tested. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Suillia affinis was found to be negative for Wolbachia. |
Water Boatman