Wolbachia Project #1 Ant

November 14, 2024

Sample information
Picture
Photos by: Bineta K.
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group Davies Career and Technical High School
Observations

The ant was found in a decaying log in the forest behind the school. It looks similar to a me at any from Australia. It has quiet a hard shell and moved too fast to take a picture of it in the log.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am highly confident because all of the controls work. Everything that was supposed to show up in gel was present and the non dna samples came up negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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