Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Davies Career and Technical High School |
| Observations | The ant was found in a decaying log in the forest behind the school. It looks similar to a me at any from Australia. It has quiet a hard shell and moved too fast to take a picture of it in the log. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am highly confident because all of the controls work. Everything that was supposed to show up in gel was present and the non dna samples came up negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid