Sample information |
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Picture |
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Location | |
Collection date | 09/19/2024 |
Captive / Cultivated? | Wild-caught |
Group | Davies Career and Technical High School |
Observations | The ant was found in a decaying log in the forest behind the school. It looks similar to a me at any from Australia. It has quiet a hard shell and moved too fast to take a picture of it in the log. |
Putative identification | Arthropoda |
Methods |
|
Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | I am highly confident because all of the controls work. Everything that was supposed to show up in gel was present and the non dna samples came up negative. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Arthropoda was found to be postive for Wolbachia. |