Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/22/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | Arthropod was collected in the backyard of a house. The backyard was grassy, had many plants growing and grown, had pear & apple trees, and the soil was a little dry. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Before being placed under UV light, the gel containing the DNA of all arthropods cracked on the ground. This means that the results of these samples may be inaccurate due to contamination and leakage of DNA.
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | The confidence level is medium because of inaccurate results. Although the (+) Arthropod control and the DNA controls (+/-) appeared as expected, the DNA ladder and the (-) Arthropod control did not appear correctly. The breakage in the gel caused inaccurate data in Arthropod #1 and Arthropod #3 although Wolbachia was present in Arthropod #2.
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |


European Paper Wasp
Woodworm Ant