Eurylister carolinus

Sample information

Picture
Entry by: Kevin N.
Location
Collection date 10/22/2024
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

Arthropod was collected in the backyard of a house. The backyard was grassy, had many plants growing and grown, had pear & apple trees, and the soil was a little dry.

Putative identification Arthropoda

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Before being placed under UV light, the gel containing the DNA of all arthropods cracked on the ground. This means that the results of these samples may be inaccurate due to contamination and leakage of DNA.

 

Results

Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

The confidence level is medium because of inaccurate results. Although the (+) Arthropod control and the DNA controls (+/-) appeared as expected, the DNA ladder and the (-) Arthropod control did not appear correctly. The breakage in the gel caused inaccurate data in Arthropod #1 and Arthropod #3 although Wolbachia was present in Arthropod #2.

 

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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