Camel Cricket C-4

November 26, 2024

Sample information
Picture
Location
Collection date 09/04/2024
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Caught in my garage, urban environment. Early fall, 74ºF. Grey and brown with longs legs including two large hind legs. Rounded body with hard shell. Pointed head with small mandibles and long vertical eyes. Long thin antennae.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: We extracted DNA from the bug matter within the abdomen of the specimen.

PCR: Everything when according to protocol.

Gel Electrophoresis, Arthropod CO1: The first lane contains the DNA ladder. The second contains the sample from the Camel Cricket (C-4). The third lane has DNA from another specimen. The fourth and fifth lanes contain positive and negative controls and the sixth lane was our water control.

Gel Electrophoresis, Wolbachia:

The first lane contains the DNA ladder. The second contains the sample from the Camel Cricket (C-4). The third lane has DNA from another specimen. The fourth and fifth lanes contain positive and negative controls and the sixth lane was our water control. The ninth lane on the far right has DNA from another specimen.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We did not make any mistakes during the extraction or gel processes for this sample. Additionally all of our controls gave the intended result.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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