Jasmine Velez- Polistes fucatus

November 26, 2024

Sample information
Picture
Photos by: Jasmine V
Location
Collection date
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

It was a warm and dry summer day of 90F. Caught in a tall grass area near Benedictine University’s parking garage.
Putative identification Arthropoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes fuscatus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

High level of confidence due to the S16 rRNA gene being present after electrophoresis.
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Polistes fuscatus was found to be postive for Wolbachia.
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