Sample information

Collected during summer, found on stone wall by house, it was 69 degrees Fahrenheit outside, urban habitat, 6 legs, no wings, short brown antenna, black eyes.

Methods

DNA Extraction:

Arthropod was easy to extract DNA from, abdomen was directed and goop was scooped into the tube

PCR: No mistakes made, but Had to spin 3 times in centrifuge, had too much liquid left at top after first and second spins. Had to spin 2 times in centrifuge after buffer AW1 was added

Gel Electrophoresis, Arthropod CO1:

The first lane of our Gel #1 contained our DNA ladder, the second lane contained our A4 sample (Spider Cricket), third lane had A5 sample (Common Moth), fourth lane had sample A6 (Brown Millipede), Lanes 5 through seven contained our positive arthropod control, our negative arthropod control and our H2O control.

Our controls worked so our results are valid.

Gel Electrophoresis, Wolbachia:

In our first gel, we had in lane one our DNA ladder, lane 2 had sample A4 (Spider Cricket), the third lane had sample A5 (Common Moth), our fourth lane had sample A6 (Brown Millipede), and then in lanes 5 through 7 had our positive Wolbachia control, negative Wolbachia control, and our H2O control. Our controls worked which means we experienced no contamination and our results are valid.

Results

All of our controls worked and we experienced no mistakes or contamination of any sort.