Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/10/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Caught by in an office space during late summer, 70º. Pale yellow, all 8 legs intact, body less than an inch in length. Urban habitat. |
| Putative identification | Arthropoda Chelicerata Arachnida |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | PCR: No known mistakes made, protocol followed. Gel electrophoresis, Arthropod CO1: C1 was the Aracneae , C2 and C3 were other arthropods. W+ was positive for Wolbachia, W- was negative for Wolbachia but still contained arthropod DNA. Water was the control and no band appeared, confirming that the samples were not contaminated with other DNA. Gel electrophoresis, Wolbachia: The Aracneae was C1, C2 was another sample, and so was C3. W+ was the only lane with a band because it was the positive control. W- had no band as it was the negative control. Water also had no band which confirmed no contamination. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We have high confidence because none of the controls showed results that were not expected. Water had no band in any of the gels, W+ and W- both had bands for the CO1 gene, and only W+ had a band for the Wolbachia gel. As far as we know, no mistakes were made in relation to this sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arachnida was found to be negative for Wolbachia. |
Water Boatman