Sample information |
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Picture |
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Location | |
Collection date | 09/17/2024 |
Captive / Cultivated? | Wild-caught |
Group | Edmund Burke School |
Observations | Caught in science lab in late summer. Spotted, orangeish color, slightly damaged wing. |
Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae |
Methods |
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Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | DNA Extraction: Arthropod was crushed easily and there were no issues throughout the extraction process. PCR: No mistakes throughout process. Gel electrophoresis, Arthropod CO1: Well C2 was the Coleoptera, W+ and W- were positive and negative for Wolbachia but still contained arthropod DNA, and H2O is water to be a control for contamination. C2 had a band between 800 and 600 base pairs, same with W+ and W-. Water had no band so there is no reason to assume the samples were crosscontaminated. Gel electrophoresis, Wolbachia: |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | The negative Wolbachia control had no band so we have no reason to believe the sample was contaminated. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Coccinellidae was found to be negative for Wolbachia. |