Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/17/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Caught in science lab in late summer. Spotted, orangeish color, slightly damaged wing. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | DNA Extraction: Arthropod was crushed easily and there were no issues throughout the extraction process. PCR: No mistakes throughout process. Gel electrophoresis, Arthropod CO1: Well C2 was the Coleoptera, W+ and W- were positive and negative for Wolbachia but still contained arthropod DNA, and H2O is water to be a control for contamination. C2 had a band between 800 and 600 base pairs, same with W+ and W-. Water had no band so there is no reason to assume the samples were crosscontaminated. Gel electrophoresis, Wolbachia: There was no band present for the C-2 lane indicating it was not positive for Wolbachia. We have no reason the believe this is false because the positive Wolbachia control and a band and the negative Wolbachia control and the the water control did not have bands. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The negative Wolbachia control had no band so we have no reason to believe the sample was contaminated. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coccinellidae was found to be negative for Wolbachia. |
Water Boatman