Lepidoptera

November 4, 2024

Sample information
Picture
Location
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date: 2/26/2024​
  • Time: 1:47 p.m.​
  • Location: ​
  • Front of Lane Library Bushes. ​
  • Compass Plaza Bushes​
  • Flowers on the side of Learning Commons​
  • Behind Library Hydrangea Plant​
  • Environment: Sunny​
  • Method: Sweeping Method
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence level is medium because I tested the DNA in the gel electrophoresis, and it tested positive for Wolbachia, but it could also be an error, and my sequence did not turn out for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
>01D_GREAA-MT_CO1F TRIM QUALITY: 20 TACTTCTTTAAGTTTATTAATTCGAACAGAATTagccatcccAGGATCTT TAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTT ATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAA TTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGTA TAAATAATATAAGATTTTGGATACTTCCTCCTTCTCTAACTTTATTAATT TCAAGAAGTATTGTAGAAAATGGAGCAGGAACAGGTTGAACAGTTTATCC TCCTCTTTCTTCTAATATTGCTCATCAAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCAGGTATTTCATCTATTTTAGGAGCTATTAAT TTTATTACTACAATTATTAATATACGAATTAAAAATTTATCATTTGATCA AATACCTTTATTTGTTTGATCTGTTGGTATTACAGCCTTATTATTACTTT TATCTTTACCAGTTTTAGCAGGAGCTATTACTATACTTTTAACTGATCGA AATTTAAATACTTCATTTTTTGATCCTGCTGGAGGAGGAGATCCAATTTT ATATCAACATTTATTTTGATTTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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