Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations |
|
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | My confidence level is medium because I tested the DNA in the gel electrophoresis, and it tested positive for Wolbachia, but it could also be an error, and my sequence did not turn out for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
>01D_GREAA-MT_CO1F TRIM QUALITY: 20 TACTTCTTTAAGTTTATTAATTCGAACAGAATTagccatcccAGGATCTT TAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTT ATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAA TTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGTA TAAATAATATAAGATTTTGGATACTTCCTCCTTCTCTAACTTTATTAATT TCAAGAAGTATTGTAGAAAATGGAGCAGGAACAGGTTGAACAGTTTATCC TCCTCTTTCTTCTAATATTGCTCATCAAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCAGGTATTTCATCTATTTTAGGAGCTATTAAT TTTATTACTACAATTATTAATATACGAATTAAAAATTTATCATTTGATCA AATACCTTTATTTGTTTGATCTGTTGGTATTACAGCCTTATTATTACTTT TATCTTTACCAGTTTTAGCAGGAGCTATTACTATACTTTTAACTGATCGA AATTTAAATACTTCATTTTTTGATCCTGCTGGAGGAGGAGATCCAATTTT ATATCAACATTTATTTTGATTTTTTGGTCACC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman