Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/27/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | 10:10 am, humid and sunny morning, caught in the high grass on the far east side of the student parking lot, caught by using the sweeping method. |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We are fairly certain that the insect we had caught, has zero traces of Wolbachia present within it. We know this because we performed a gel electrophoresis test and our results came back with only one band present in the insect gene, and nothing for the Wolbachia gene. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid