bro_MGB Diptera

November 5, 2024

Sample information
Picture
Photos by: Mary B.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Field by the retention pond

GA Southern Armstrong Campus

warm weather post-rain

sweep net, forceps, mason jar
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked. The Wolbachia 16s rRNA band was not shown in the bro_MGB lane. Based on the DNA sequencing done in lab, the Wolbachia DNA was not detected.
Wolbachia 16S sequence
Arthropod COI sequence
TtngGaACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGG ATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATG CTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTT GGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCC TCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTC TTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTA TATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTT AGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTG TTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTT GATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACT TCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAG ATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCT ATTTTATACCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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