Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/27/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Environment: Dry grass in a very green area with lots of fields and bushes Temperature: Hot and humid Time: 1:05 in the afternoon |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | My confidence level in the accuracy of my experiment is high because there was many steps and procedures we followed that prevented any mistakes during the process of the PCR. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AGTATATTAATTCGAACAGAATTAAgccaTCCAGGATTTTTTATTGGTGA TGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTT TTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTT CCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATAT AAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCA TAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATCCCCCTCTTTCT AGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCT TCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTA CAGTTATTAATATGCGATCTACTGGAATTACATTTGATCGTATACCACTT TTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCC AGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATA CAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTTATACCAACAT TTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Diptera was found to be negative for Wolbachia. |
NIU17
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