DEMAHF24-ELC-Hemiptera

November 5, 2024

Sample information
Picture
Photos by: Eva C.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • collected by skimming a pond with a D-net
  • pond surrounded by trees and grass; full of other organisms (bugs, snails, etc)
  • collected around 1:15pm
Putative identification Arthropoda Hexapoda Insecta Hemiptera Nepidae

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I had predicted my sample would be positive for Wolbachia, but it was not. I was confident in my skills to carry out the procedure correctly, but my unsupportive results dampened my confidence a bit.
Wolbachia 16S sequence
Arthropod COI sequence
AGGAACTTCCTTAAGATGACTTATCCGAATCGAACtacgcctgcCAGGAT CATTCATTGGTGATGATCAAATTTATAATGTAGTAGTAACTGCCCATGCA TTCATTATAATCTTTTTTATAGTGATGCCAATTATGATCGGAGGATTTGG TAACTGACTAGTTCCTTTAATACTGGGGGCCCCAGATATAGCATTCCCAC GAATAAATAACATAAGATTCTGATTACTGCCCCCCTCACTAACTCTTCTT TTATCAAGTAGAATCGCCGAAAGAGGGGCCGGAACTGGATGAACAGTATA TCCTCCCCTATCTACTAATATTTCACACAGTGGAGCATCCGTAGATTTAG CTATTTTTTCATTACATTTAGCTGGTGTTTCCTCAATTTTAGGTGCTGTT AATTTTATTTCAACAATTATTAACATGCGAGCCACAGGAATAACCTCCGA CCGAATACCTTTATTTGTATGATCCGTAGGTATTACCGCCCTTCTTTTAC TTTTATCACTACCTGTACTTGCCGGAGCAATTACTATACTATTAACAGAT CGAAATTTAAATACCTCATTTTTCGATCCAGCAGGAGGAGGGGACCCAAT TCTTTATCAACACTTGTTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Nepidae was found to be negative for Wolbachia.
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