DEMAHF24-ACB Diptera

November 7, 2024

Sample information
Picture
Photos by: Andrew B.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught Aug 27, 2024 from 1:00 to 1:20 PM. Collected in tall grass near a creek.
Putative identification Arthropoda Hexapoda Insecta Diptera Bibionidae Plecia Plecia nearctica

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis showed the lack of a second band for the sample, indicating that the sample was negative for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
AACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGGATTTT TTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTT ATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAA CTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAA TAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTA ATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATCC CCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAAT TTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTTGATCG TATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCC TATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCGA AATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTT ATACCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Plecia nearctica was found to be negative for Wolbachia.
Report Inappropriate Post