Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/27/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught Aug 27, 2024 from 1:00 to 1:20 PM. Collected in tall grass near a creek. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Bibionidae Plecia Plecia nearctica |
Methods |
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| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
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| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The gel electrophoresis showed the lack of a second band for the sample, indicating that the sample was negative for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGGATTTT TTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTT ATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAA CTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAA TAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTA ATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATCC CCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAAT TTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTTGATCG TATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCC TATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCGA AATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTT ATACCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Plecia nearctica was found to be negative for Wolbachia. |
Water Boatman