DEMAHF24-MCT Hemiptera

November 7, 2024

Sample information
Picture
Photos by: McKenzie T.
Location No map location data available.
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insect was in the grass along with other fallen leaves.​ The sweep net method was used to capture insect.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I feel that I can trust my results because the controls worked. I ended up with two bands showing which indicates that my insect was positive of Wolbachia. 
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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