Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Davies Career and Technical High School |
| Observations | It was hopping in a feild near a different building. It was caught while resting |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The bug doesn’t have Wolbachia but it showed up in the control. My arthropod had no band which means the grasshopper did not have Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Water Boatman