Banded Longhorn Beetle

April 9, 2025

Sample information
Picture
Photos by: Nithil V.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The larvae was burrowed into a piece of dead wood.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Cerambycidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Larva
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My sample is in lane 5.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

On the Wolbachia gel there was no bar at 438 base pairs for lane 5, indicating that there was no Wolbachia DNA present in my sample.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
NNNNNNNNNNATTTTTGGAGCNTGAGCAGGNATANTTGGAACTTCTCTAAGACTCCTAATTCGTACAGAACTAGGAAACCCTGGATCTTTAATTGGTGACGATCAAATTTATAATGTTATTGTTACCGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATCGGGGGGTTTGGAAATTGATTAGTTCCCTTAATATTAGGAGCACCAGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGATTACTTCCTCCCTCATTAACATTATTAATTATAAGAAGAATTGTAGAAAGAGGGGCTGGTACAGGATGAACTGTATACCCACCCCTATCTTCTAACATTGCTCACGGAGGTTCTTCTGTTGATTTGGCTATTTTCAGTCTTCATTTAGCAGGTATTTCTTCCATTTTAGGGGCAGTTAACTTTATCACTACAGTTATTAATATACGACCAGTTGGAATAAGACCAGATCGAATACCTCTCTTTGTTTGAGCTGTTGTAATTACTGCTGTTCTTTTATTACTCTCTTTACCTGTCTTAGCAGGGGCAATTACTATACTTTTAACAGATCGTAATCTTAATACTTCATTTTTTGATCCAGCTGGTGGGGGAGACCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCNCNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Cerambycidae was found to be negative for Wolbachia.
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