Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Nylanderia Nylanderia fulva |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Our controls did not work for DNA extraction indicating as a group, we had inconsistencies extracting sufficient DNA for PCR. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
(no title)
Arthropod Collection Lab
House Cricket
Week 2/18 lab – Arthropod Collection
Week 2/18 Bio Lab Arthropod Identification