Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Moved around very fast when tried to catch the ant; orangish color; has dark spots on back of body; black eyes. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Pogonomyrmex Pogonomyrmex occidentalis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | We pipetted the samples into the gel wells and added buffer. We performed the procedure correctly; no bands were present in the gel. The sample was not sent for sequencing. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | I do not know the results to my arthropods because my group did not send our findings. |
| Wolbachia 16S sequence |
n/a
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
n/a
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid