Joro Spider

November 17, 2024

Sample information
Picture
Photos by: Anne R.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Found on a spider web between two trees in the Walton outdoor classroom space. 

  • Temp: 12.1°C 
  • Longitude: 33°59’18’’N 
  • Latitude: 84°26’19’’W
Putative identification Arthropoda Chelicerata Arachnida Araneae Nephilidae Trichonephila Trichonephila clavata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The DNA PCR was not successful because the bands did not correlate to the Wolbachia control band.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The confidence level is low because the controls were not present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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