Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | spider, yellow and black on legs, taken off of its web, small maybe few millimeters, very active while in captivity |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Araneidae Mangora Mangora placida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | gel electrophoresis was run, we did not get good results on any of them. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | There are not any bright bands when there should have been at least some for the controls. We think the problem was with the DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Oncopeltus fasciatus
Formica Pallidefulva
Hylephila Phyleus
Thomisus onustus
Halictus Ligatus