Ant

November 18, 2024

Sample information
Picture
Photos by: Soshi Kusanagi
Location
Collection date 11/14/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Brownish in color, with a tint of red on the head. The abdomen has some black as well.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Lane 11

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Control groups did not group, positive did not show up and the negative showed up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius was found to be negative for Wolbachia.
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