Sample information |
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Picture |
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Location | |
Collection date | 11/12/2024 |
Captive / Cultivated? | Wild-caught |
Group | Walton High School |
Observations | The baby termite was found under a tree bark near the creek. It seems to be extremely small and has a soft body. They seem to lack an exoskeleton and they are white-cream colored. They lack wings and they have antennae. |
Putative identification | Arthropoda Hexapoda Insecta Blattodea Rhinotermitidae Coptotermes Coptotermes formosanus |
Methods |
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Extraction kit | tweezers |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniPCR |
Buffer | 0.25 TAE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | Low |
Explanation of confidence level | I have low confidence because some of the controls failed to even work, meaning there could have been some experimental error. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Coptotermes formosanus was found to be negative for Wolbachia. |