Flat-Backed Millipede

April 3, 2025

Sample information
Picture
Photos by: Subish A.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The flat-backed millipede was found under a rock near a creek squirming around rapidly. They have flattened bodies and a segmented body with many, many legs. They also seem to have small antennae. They have a grey earthy color with bits of orange.
Putative identification Arthropoda Myriapoda Diplopoda Polydesmida

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 0.25 TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I have low confidence for this because some of the controls did not even work meaning there could have been some experimental error that could have occurred during our gel process.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polydesmida was found to be negative for Wolbachia.
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