Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | The flat-backed millipede was found under a rock near a creek squirming around rapidly. They have flattened bodies and a segmented body with many, many legs. They also seem to have small antennae. They have a grey earthy color with bits of orange. |
| Putative identification | Arthropoda Myriapoda Diplopoda Polydesmida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | There was no visible band for the sample. The sample was not sent for sequencing. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | I have low confidence for this because some of the controls did not even work meaning there could have been some experimental error that could have occurred during our gel process. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Baetis21Cb2.1
Diptera21Aa2.1
Baetis21Aa6
Baetis21Aa2.3
Diptera_Cb6