Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | Approximately 1 cm in length. Brown/orange limbs with darker brown/black head, wings, and abdomen. Is able to fly. Attracted to lights. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Oedemeridae Ananca Ananca bicolor |
Methods |
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| Extraction kit | pcr |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | 2% agarose gel with a 1kb DNA ladder loaded into lane 1 |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The positive controls (b.2, c.4) and negative controls (b.3, c.3) both worked, and the Ananca bicolor sample tested positive for Arthropod CO1 (e.2, e.3), so the DNA extraction for the A. bicolor samples was successful.
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| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Ananca bicolor was found to be negative for Wolbachia. |
Water Boatman