False Blister Beetle (Ananca bicolor)

November 25, 2024

Sample information
Picture
Photos by: JordanM
Location
Collection date 11/14/2024
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Approximately 1 cm in length. Brown/orange limbs with darker brown/black head, wings, and abdomen. Is able to fly. Attracted to lights.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Oedemeridae Ananca Ananca bicolor

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

2% agarose gel with a 1kb DNA ladder loaded into lane 1

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The positive controls (b.2, c.4) and negative controls (b.3, c.3) both worked, and the Ananca bicolor sample tested positive for Arthropod CO1 (e.2, e.3), so the DNA extraction for the A. bicolor samples was successful.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ananca bicolor was found to be negative for Wolbachia.
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