False Blister Beetle (Ananca bicolor)

November 25, 2024

Sample information
Picture
Photos by: JordanM
Location
Collection date 11/14/2024
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Approximately 1 cm in length. Brown/orange limbs with darker brown/black head, wings, and abdomen. Is able to fly. Attracted to lights.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Oedemeridae Ananca Ananca bicolor

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

CO1 arthropod 708 showed up on the gel for both samples so I think that I managed to extract the DNA properly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ananca bicolor was found to be negative for Wolbachia.
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