Sample information |
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Picture |
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Location | |
Collection date | 10/06/2024 |
Captive / Cultivated? | Wild-caught |
Group | Portland State University |
Observations | |
Putative identification | Arthropoda Hexapoda Insecta Hemiptera Coreidae Leptoglossus Leptoglossus occidentalis |
Methods |
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Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Partial abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | |
Buffer | |
DNA stain | |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Gel A, Wells 5-7. Well 5 is positive for arthropod DNA, well 6 is positive for the Wolbachia positive control, and well 7 is negative for the insect’s Wolbachia test. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Leptoglossus occidentalis was found to be negative for Wolbachia. |